The protection of human rights and fundamental freedoms, including the rights of women and girls, together with the support of democracy, continue to be among the core objectives of the Czech Republic's foreign policy. In accordance with the Concept of Human Rights Support and Transformational Cooperation, the Czech Republic supports equal opportunities for women and men in international organizations and actively opposes violence against women and girls, including sexual violence in armed conflicts, at meetings of the UN and other international organizations. In this regard, the Czech Republic also supports the activities of UN WOMEN and contributes to the fulfillment of EU guidelines on violence against women and girls and the fight against all forms of discrimination against them. As part of the Universal Periodic Review of the UN Human Rights Council, the Czech Republic formulates, among other things, recommendations focused on sexual abuse and trafficking in women, gender discrimination, the eradication of female circumcision, or the fight against sexual and gender-based violence, including domestic violence.

In 2023, within the UN Human Rights Council, the Czech Republic actively supported the adoption of resolutions to combat violence against women, with a focus on criminally detained women and child, early and forced marriages. The Czech Republic also supported the adoption of the resolution on the right to education for girls. The Czech Republic co-sponsored a resolution on preventing maternal mortality. In the 3rd Committee of the UN Security Council, the Czech Republic supports the resolution on the improvement of the situation of women and girls in rural areas.